Entire Time Table

October 17 Mon
1400- On-site registration
1500- Hotel check-in
1610-1755 Welcome reception
1800-2100 Opening sessions
2100-2300 Lounge time

October 18 Tue
0820-0830 Welcome remarks
0830-1200 Session: Peripheral oxygen sensing and respiratory control (210 min)
1210-1320 Lunch
1320-1450 Poster session I (90 min)
1500-1810 Session: Central CO2/pH sensing and respiratory control (190 min)
1830-1940 Dinner
1950-2050 Valedictory lecture I by Jeffrey Smith (60 min)
2100-2300 Lounge time (Mini party to celebrate Jeff’s happy retirement)

October 19 Wed
0820-1045 Session: Respiratory rhythm generation I (145 min)
1100-1200 Keynote lecture by Jack Feldman (60 min)
1210-1320 Lunch
1320-1450 Poster session II (90 min)
1500-1720 Session: Spinal cord and respiratory control (140 min)
1725-1825 Invited special lecture by Hideyuki Okano (60 min)
1830-1940 Dinner
1950-2050 Valedictory lecture II by Patrice Guyenet (60 min)
2100-2300 Lounge time (Mini party to celebrate Patrice’s happy retirement)

October 20 Thu
0820-1110 Session: Respiratory control by higher brain and dyspnea sensation (170 min)
1120-1150 Group photo
1200-1310 Lunch
1320-1610 Session: Control of upper airway movement during awakefulness/sleep and swallowing (170 min)
1620-1920 Session: Respiratory rhythm generation II (180 min)
1930-2110 Gala dinner
2120-2300 Lounge time

October 21 Fri
0800-1010 Session: Interaction of respiratory and cardiovascular control systems (130 min)
1020-1230 Session: Central oxygen sensing and respiratory control (130 min)
1240-1300 Closing remarks and announcement of journal special issues etc.
1300 Farewell
1320 Bus departure for Odawara Station
1330 Bus departure for Tokyo Station (Tokyo Terminal, Marunouchi side) via Odawara Station

Scientific Sessions (Oral)

Keynote lecture: October 19 Wed
Chairperson 1: Yasumasa Okada (Japan)
Chairperson 2: Patrice Guyenet (USA)
Speaker (50+10 min): Jack Feldman, University of California at Los Angeles, Los Angeles, CA, USA; Our Future in Breathing

Valedictory lecture I: October 18 Tue
Chairperson 1: Naohiro Koshiya (USA)
Chairperson 2: Hidehiko Koizumi (USA)
Speaker (50+10 min): Jeffrey Smith, National Institutes of Health, Bethesda, MD, USA, Discovering the Origin of Respiratory Rhythm: Looking Back and Forward

Valedictory lecture II; October 19 Wed
Chairperson 1: Chikara Abe (Japan)
Chairperson 2: Tsuyoshi Inoue (Japan)
Speaker (50+10 min): Patrice Guyenet, University of Virginia, Charlottesville, VA, USA, The retrotrapezoid nucleus, lynchpin of CO2 homeostasis

Invited special lecture: October 19 Wed
Chairperson 1: Gordon Mitchell (USA)
Chairperson 2: Lyandysha Zholudeva (USA)
Speaker (50+10 min): Hideyuki Okano, Keio University, Tokyo, Japan, Regeneration of the injured spinal cord using iPSCs-based cell therapies

October 17 Mon

Opening session 1: Pathophysiology of respiratory control (45+5 min=45 min talk & discussion followed by 5 min break)
Chairperson 1: Luciane Helena Gargaglioni Batalhão (Brazil)
Chairperson 2: Michael Lane (USA)
Speaker 1 (15+5 min): Yingtang Shi, University of Virginia, Charlottesville, VA, USA, A brainstem peptide system activated at birth protects postnatal breathing
Speaker 2 (20+5 min): Ken O’Halloran, University College Cork, Cork, Ireland, Control of breathing in a mouse model of muscular dystrophy

Opening session 2: Lung and airway (70+10 min)
Chairperson 1: Yingtang Shi (USA)
Chairperson 2: Jerry Yu (USA)
Speaker 1 (20+5 min): Jerry Yu, University of Louisville and Robley Rex VAMC, KY, USA, Multiple sensor theory in airway mechanosensory units
Invited lecture Speaker (40+5 min): Mitsuru Morimoto, Center for Biosystems Dynamics Research, RIKEN, Kobe, Japan, Lung development in mouse and human

Opening session 3: Modelling of the respiratory control system (50 min)
Chairperson 1: Brian J. Dlouhy (USA)
Chairperson 2: Consuelo Morgado-Valle (México)
Speaker 1 (15+5 min): Christopher G. Wilson, Loma Linda University, Loma Linda CA USA, COVID-19 and silent hypoxemia in a minimal closed-loop model of the respiratory oscillator
Speaker 2 (25+5 min): Ryan Phillips, Seattle Children’s Research Institute, Seattle WA, USA, Interdependence of cellular and network properties in respiratory rhythmogenesis

October 18 Tue

Session: Peripheral oxygen sensing and respiratory control (190+20=210 min)
Chairperson 1: Jose Lopez-Barneo (Spain)
Chairperson 2: Mieczyslaw Pokorski (Poland)
Plenary lecture 1 (40+5 min): Jose Lopez-Barneo (University of Seville, Seville, Spain) Molecular mechanisms of acute oxygen sensing by arterial chemoreceptors
Plenary lecture 2 (40+5 min): Nanduri Prabhakar (The University of Chicago, Chicago, USA) O2 sensing by the carotid body in health and disease
Speaker 1 (15+5 min): Rodrigo Iturriaga (Catholic University of Chile, Santiago de Chile, Chile) Carotid body overactivation. Sympathetic overflow. Effects of carotid body denervation.
Speaker 2 (15+5 min): Mieczyslaw Pokorski (Opole University, Opole, Poland), Expression of p11 and heteromeric TASK channels in rat carotid chemoreceptor cells
Speaker 3 (15+5 min): Camillo Di Giulio (Chieti University, Chieti, Italy) Aging carotid body.
Speaker 4 (15+5 min): Silvia Conde (Nova University of Lisbon, Lisbon, Portugal), Metabolic syndrome and carotid body overactivation. Inhibition of carotid body output
Speaker 5 (15+5 min): Alfredo Garcia (The University of Chicago, Chicago, USA), Carotid body-dependent remodeling of hippocampal function by intermittent hypoxia.

Session: Central CO2/pH sensing and respiratory control (170+20=190 min)
Chairperson 1: George Richerson (USA)
Chairperson 2: Douglas Bayliss (USA)
Plenary lecture 1 (40+5 min): George Richerson, University of Iowa, Iowa City, IA, USA, Comparison of chemosensitivity of acutely dissociated 5-HT and RTN neurons
Plenary lecture 2 (40+5 min): Douglas Bayliss, University of Virginia, Charlottesville, VA, USA, Molecular and cellular basis for RTN-mediated respiratory chemoreception
Speaker 1 (15+5 min): Jaime Eugenín, Universidad de Santiago de Chile, Santiago, Chile, Glial contribution to Central Respiratory Chemoreception
Speaker 2 (15+5 min): Russell Ray, Baylor College of Medicine, Houston, TX, USA, The role of central noradrenergic – glutamatergic co-transmission in respiratory homeostasis.
Speaker 3 (15+5 min): Luciane Helena Gargaglioni Batalhão, São Paulo State University, São Paulo, Brazil, Locus coeruleus contribution to behavioral responses elicited by CO2
Speaker 4 (15+5 min): Daniel Mulkey, University of Connecticut, Storrs, CT, USA, H+-dependent myo-inositol cotransport preferentially regulates activity of RTN chemoreceptors by a Kcnq2-dependent mechanism

October 19 Wed

Session: Respiratory rhythm generation I (125+20=145 min)
Chairperson 1: Hiroshi Onimaru (Japan)
Chairperson 2: Daniel Mulkey (USA)
Speaker 1 (20+5 min): Keiko Ikeda, Showa University School of Dentistry, Tokyo, Japan, Cell characterization of the pFRG and local neuron network analysis of medullary respiratory center in newborn rat
Speaker 2 (15+5 min): Silvia Pagliardini, University of Alberta, Edmonton, Canada, Expiratory activity in sleep states
Speaker 3 (15+5 min): Robert Huckstep, School of Life Sciences, University of Warwick, Coventry, UK, Analysing neuronal activity in rostral brainstem circuits and their response to CO2
Speaker 4 (15+5 min): Consuelo Morgado-Valle, Universidad Veracruzana, Xalapa, Veracruz, México, Regulation of inspiratory burst frequency, amplitude, and duration in vitro
Speaker 5 (15+5 min): George Souza, School of Medicine, University of Virginia, Charlottesville, USA, Influence of respiratory chemoreceptors on the stability of breathing across vigilance states
Speaker 6 (15+5 min): Swen Hülsmann, University Hospital Göttingen, Göttingen, Germany, GABA/Glycine co-transmission in the respiratory network

Session: Spinal cord and respiratory control (125+15=140 min)
Chairperson 1: Gordon Mitchell (USA)
Chairperson 2: Lyandysha Zholudeva (USA)
Plenary lecture (40+5 min): Gordon Mitchell (University of Florida, Gainesville, FL, USA), Mechanisms and implications of phrenic motor plasticity
Speaker 1 (15+5 min): Tracy L. Baker (University of Wisconsin, Madison, WI, USA), Fear of failure: mechanisms of spinal neuroplasticity induced by central apneas
Speaker 2 (15+5 min): Carlos B. Mantilla (Mayo Clinic, Rochester, MN, USA), Neurotrophic mechanisms of recovery of respiratory function following cervical spinal cord injury
Speaker 3 (15+5 min): Erica A. Dale (University of Florida, Gainesville, FL, USA), Epidural stimulation for recovery of respiration after spinal cord injury
Speaker 4 (15+5 min): Lyandysha V. Zholudeva (Gladstone Institutes, San Francisco, CA, USA), Transplanted human spinal interneurons functionally integrate with the injured phrenic network

October 20 Thu

Session: Respiratory control by higher brain and dyspnea sensation (135+20=155 min)
Chairperson 1: Yuri Masaoka (Japan)
Chairperson 2: Paul Davenport (USA)
Plenary lecture 1 (30+5 min): Paul Davenport, University of Florida, Gainesville, Florida, USA, The Mechanisms of Perception of Effort Elicited Dyspnea
Speaker 1 (20+5 min): Brian J. Dlouhy, University of Iowa, Iowa City, Iowa, USA, An Amygdala Site that Inhibits Breathing and Dyspnea
Speaker 2 (20+5 min): Yuri Masaoka, Showa University School of Medicine, Tokyo, Japan, Emotional and Cognitive Aspects of Breathing Explored in Relation to Olfaction
Speaker 3 (20+5 min): Nozomu H. Nakamura, Hyogo Medical University, Nishinomiya, Japan, Respiration timing-dependent modulation of neural substrates during cognitive processes.
Speaker 4 (20+5 min): Nicholas Napoli, University of Florida, Gainesville, Florida, USA, Non-Sinusoidal Analysis of Breathing for Improved Breathing Models

Session: Control of upper airway movement during wakefulness/sleep and swallowing (155+15=170 min)
Chairperson 1: Yoshitaka Oku (Japan)
Chairperson 2: Mathias Dutschmann (Australia)
Speaker 1 (20+5 min): Mathias Dutschmann, Florey Institute of Neuroscience and Mental Health, Melbourne, Australia, Distributed network mechanisms that generate the postinspiratory laryngeal adduction and orofacial behavior.
Speaker 2(15+5 min): Teresa Pitts, University of Louisville, Louisville, USA, Down & back again: the spinal cord may lead the way to laryngeal regulation.
Speaker 3 (15+5 min): Yoichiro Sugiyama, Kyoto Prefectural University of Medicine, Kyoto, Japan, Neuronal characteristics and sensorimotor coordination of swallowing central pattern generator.
Speaker 4 (15+5 min): Bowen Dempsey, Macquarie University, Sydney, Australia, A medullary centre for lapping.
Speaker 5 (15+5 min): Alyssa Huff, Seattle Children’s Research Institute, Seattle, USA Unraveling swallow-breathing interactions using in vivo optogenetic techniques.
Speaker 6 (20+5 min): Peter Burke, Macquarie University, Sydney, Australia, Nocturnal swallowing augments the arousal intensity and arousal tachycardia.
Speaker 7 (20+5 min): Yoshitaka Oku, Hyogo Medical University, Nishinomiya, Japan, Breathing-swallowing interactions and exacerbation of respiratory diseases.

Session: Respiratory rhythm generation II (160+20=180 min)
Chairperson 1: Christopher Del Negro (USA)
Chairperson 2: Jan-Marino (Nino) Ramirez (USA)
Speaker 1 (15+5 min): Nathan Baertsch, University of Washington, Seattle, WA, USA, Parabrachial tachykinin1 neurons control state-dependent breathing patterns
Speaker 2 (15+5 min): Donatella Mutolo, Università degli Studi di Firenze, Firenze, Italy, The role of preBötzinger Complex in opioid-induced respiratory depression in the adult rabbit
Speaker 3 (15+5 min): Rishi Dhingra, Florey Institute of Neuroscience and Mental Health, Melbourne, Australia, Breathing pattern generation via associative memory
Speaker 4 (15+5 min): Sufyan Ashhad, University of California at Los Angeles, Los Angeles, CA, USA, preBötzinger Complex synchronization dynamics
Speaker 5 (15+5 min): Kevin Yackle, University of California at San Francisco, San Francisco, CA, USA, A momentary “go” signal is sufficient to elicit complete vocalization
Speaker 6 (25+5 min): Jan-Marino Ramirez, University of Washington, Seattle, WA, USA, Spatiotemporal dynamics of the Respiratory Network
Speaker 7 (25+5 min): Christopher A. Del Negro, William & Mary, Williamsburg, VA, USA, Role of persistent Na+ and mixed cationic currents in breathing rhythmogenesis

October 21 Fri

Session: Interaction of respiratory and cardiovascular control systems (120+10=130 min)
Chairperson 1: Tomoyuki Kuwaki (Japan)
Chairperson 2: Rodrigo Del Rio (Chile)
Plenary lecture (40+5 min): Tomoyuki Kuwaki, Kagoshima University, Kagoshima, Japan Medullary raphe serotonergic neuron regulates stress-induced cardiorespiratory excitation
Speaker 1 (20+5 min): Rodrigo Del Rio, Pontificia Universidad Católica de Chile, Santiago, Chile, Medullary C1 pre-sympathetic neurons and their role in cardiorespiratory dysfunction in heart failure
Speaker 2 (20+5 min): Clément Menuet, INMED, INSERM, Aix-Marseille University, Marseille, France, A Hypothalamus-preBötzinger Complex-Nucleus Ambiguus network for oxytocinergic amplification of respiratory sinus arrhythmia.
Speaker 3 (20+5 min): Tadachika Koganezawa University of Tsukuba, Tsukuba, Japan, Functional roles of endogenous hydrogen sulfide in the respiratory and cardiovascular control systems

Session: Central oxygen sensing and respiratory control (120+10=130 min)
Chairperson 1: Richard Wilson (Canada)
Chairperson 2: Tara Janes, (Canada)
Plenary lecture (40+5 min): Richard Wilson, University of Calgary, Calgary, Canada, Breathing is back! Contribution of spinal oxygen sensors to ventilation and cardiovascular control
Speaker 1 (20+5 min): Greg Funk, University of Alberta, Edmonton, Canada, Postnatal development of glial purinergic signaling in the preBotC and its contribution to the development of the hypoxic ventilatory response
Speaker 2 (20+5 min): Akito Nakao, Kyoto University, Kyoto, Japan, The role of TRPA1 channel in hypoxia sensing
Speaker 3 (20+5 min): Tara Janes, Université Laval, Québec, Canada, The “nature” of Bullfrog respiratory development: central hypoxia reveals a robust motor command for air breathing prior to metamorphosis

Poster Sessions

Posters are classified into4 groups; Group 1 (P01-P13), Group 2 (P14-P26), Group 3 (P27-P38) and Group 4 (P39-P50). In each group at the beginning of each poster session, please introduce the important point of the poster within 2.5 min (max 3 min) without discussion one by one in a sequential order. Please follow the instructions from the chairpersons.

Poster I (90 min) October 18 Tue (P01 – P26)

P01
Modulation of swallowing patterns using Gaussian stochastic mechanosensory stimulation
In Morimoto1, Michael Frazure2, Kimberly Iceman2, Takuji Koike1, Teresa Pitts2
1Department of Mechanical and Intelligent Systems Engineering, The University of Electro-Communications, Tokyo, Japan, 2Department of Neurological Surgery, Kentucky Spinal Cord Injury Research Center, University of Louisville, Louisville, Kentucky

P02
Activation of Phox2b neurons in the dorsal medulla induced sucking and hiccup movement
Makito Iizuka1, Keiko Ikeda1, Hiroyuki Igarashi2, Kazuto Kobayashi3, Hiroshi Onimaru1, Masahiko Izumizaki1
1 Dept Physiol, Showa Univ Sch Med, 2, Dept Physiol Pharmacol, Schulich Sch Med Dent, Robarts Res Inst, Univ Western Ontario, 3, Dept Mol Gen, Inst Biomed Sci, Fukushima Med Univ Sch Med,

P03
Altered cardiorespiratory function in Alzheimer’s disease; characterizing sleep-disordered breathing and autonomic cardiac imbalance in APP/PS1 transgenic mice.
Camilo Toledo1, Karla G. Schwarz1, Alejandra Álvarez2, Nibaldo C. Inestrosa2,3, Rodrigo Del Rio1,2,3
1Laboratory of Cardiorespiratory Control, Department of Physiology, Pontificia Universidad Católica de Chile, Santiago, Chile. 2Center for Aging and Regeneration (CARE-UC), Pontificia Universidad Católica de Chile, Santiago, Chile. 3Centro de Excelencia de Biomedicina de Magallanes (CEBIMA), Universidad de Magallanes, Punta Arenas, Chile.

P04
Respiratory sinus arrhythmia of spontaneously breathing un-anesthetized newborn and adult rats
Nana Sato Hashizume1), Yoichiro Kitajima1), Ryoji Ide1), Chikako Saiki1)
1) Department of Physiology, The Nippon Dental University, School of Life Dentistry at Tokyo.

P05
Effects of disinhibition of the medial prefrontal cortex on cardiopulmonary output in anesthetized mice
Zachary T. Glovak1, Nicholas E. Bush1, Jan-Marino Ramirez1, 2, 3
1Seattle Children’s Research Institute, Seattle, WA, USA.2Department of Pediatrics, University of Washington, Seattle, WA, USA. 3Department of Neurological Surgery, University of Washington, Seattle, WA, USA

P06
Possible roles of Parabrachial nucleus for nociception-respiration relay control
Akiko Arata1, Naoko Masutani1, Yuki Kosaka1, Hirotaka Ooka1, Sotatsu Tonomura2, Koichi Noguchi3
1Div. of Physiome, Dept. Physiol. Hyogo Medical University, 2Dept. of Anatomy, Kawasaki Medical School, 3Dept. Anatomy, Hyogo Medical University, Japan.

P07
PiCo and preBötC neurons contribute to respiratory-sympathetic coupling in control and CIH mice
Marlusa Karlen-Amarante1, Alyssa Huff1, Luiz M Oliveira1 and Jan-Marino Ramirez1,2
1 Center for Integrative Brain Research, Seattle Children’s Research Institute. Seattle, WA, USA. 2 Department of Neurological Surgery, University of Washington, Seattle WA, USA.

P08
Opioids Impact Feeding Behavior and Response to Aerodigestive Afferent Stimulation
Michael Frazure1,2 Clinton Greene1, Kimberly Iceman1, Teresa Pitts1
1Department of Neurological Surgery and Kentucky Spinal Cord Injury Research Center, School of Medicine, University of Louisville, Louisville, KY, USA. 2Department of Physiology, School of Medicine, University of Louisville, Louisville, KY, USA.

P09
Oxytocin receptor activation in the parafacial respiratory region improves opioid induced respiratory depression
Emmanuel V. Araújo1, Ana C. Takakura2, Thiago S. Moreira1.
1 Department of Physiology and Biophysics; Institute of Biomedical Sciences, University of Sao Paulo, São Paulo, Brazil. 2 Department of Pharmacology; Institute of Biomedical Sciences, University of Sao Paulo, São Paulo, Brazil

P10
Large-scale population dynamics of the ventral respiratory column during normal breathing, opioid-induced respiratory depression, and gasping
Nicholas E. Bush1, Jan-Marino Ramirez1,2,3
1Seattle Children’s Research Institute, Seattle WA USA. 2Department of Pediatrics, University of Washington, Seattle WA USA. 3Department of Neurological Surgery, University of Washington Seattle WA USA

P11
Dynamic Rhythmogenic Network States Drive Differential Opioid Responses in the Respiratory Network.
Nicholas Burgraff1, Nicholas Bush1, Nathan Baertsch1,2, Jan-Marino Ramirez1,3.
Center for Integrative Brain Research, Seattle Children’s1, Department of Pediatrics, University of Washington2, Department of Neurological Surgery, University of Washington3

P12
Effects of Cisplatin on Respiratory Activity in Neonatal Rats
Shinichiro Ota, Hiroshi Onimaru, Masahiko Izumizaki
Department of Physiology, Showa University School of Medicine

P13
Chemical organization of solitary nucleus neurons activated by vagal afferents from the respiratory tracts
Shigefumi Yokota1, Kotaro Takeda2, Noriyuki Hama3, Yasumasa Okada4
1Department of Anatomy and Neuroscience, Shimane University School of Medicine, Izumo, Japan. 2Faculty of Rehabilitation, School of Health Sciences, Fujita Health University, Toyoake, Japan. 3Department of Neural and muscular physiology, Shimane University School of Medicine, Izumo, Japan. 4Clinical Research Center, Murayama Medical Center, Tokyo, Japan

P14
Etonogestrel, a progestin-based drug, rescues ventilation in an in vivo rodent model of central CO2-chemoreflex impairment
Tara A. Janes1,2, Silvia Cardani1,2 and Silvia Pagliardini1,2,3
1Department of Physiology, 2Women and Children’s Health Research Institute, 3Neuroscience and Mental Health Institute, University of Alberta, Edmonton, Alberta, Canada.

P15
Role of olfactory chemoreceptors in the hypercapnic ventilatory response depression
Phelipe E. Silva1, Cleyton R. Sobrinho1, Ana C. Takakura2, Thiago S. Moreira1.
1 Department of Physiology and Biophysics; Institute of Biomedical Sciences, University of Sao Paulo, São Paulo, Brazil.2 Department of Pharmacology; Institute of Biomedical Sciences, University of Sao Paulo, São Paulo, Brazil

P16
Influence of respiratory chemoreceptors on breathing stability across vigilance states in adult mice
George MPR Souza, Daniel S Stornetta, Douglas A Bayliss and Stephen BG Abbott
Department of Pharmacology, University of Virginia, Charlottesville, VA

P17
Microglia phenotype modification by hypercapnia
Rommy von Bernhardi1, Sebastián Beltrán-Castillo2,3, Estefanía Irribarra2, Jaime Eugenín2
1Faculty of Medicine and Science, Universidad San Sebastián, Santiago, Chile. 2Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile. 3Centro integrativo de Biología y Química Aplicada (CIBQA), Universidad Bernardo O’Higgins, Santiago, Chile.

P18
The contribution of Ca2+-activated K+ (BK) channels on CO2 chemosensitivity
Luis Gustavo A. Patrone1, Christine A. Ratliff2, Luciane H. Gargaglioni1
1Dept. of Animal Morphology and Physiology, Sao Paulo State University, FCAV, Jaboticabal, SP, Brazil. 2Department of Neuroscience, Cell Biology and Physiology, Wright State University Boonshoft School of Medicine, Dayton, Ohio

P19
Cell-type Specific Differential Gene Expression due to Chronic Intermittent Hypoxia (CIH) in the Pons and Medulla of Adult Mice Revealed by Single-Nuclei RNA-Seq
Hemalatha Bhagavan1, Aguan D. Wei1, Alyssa Huff1, and Jan-Marino Ramirez1,2
1Center for Integrative Brain Research, Seattle Children’s Research Institute, Seattle, WA, USA. 2Department of Neurological Surgery, University of Washington, Seattle, WA, USA.

P20
The role of Phox2b in the respiratory network
Silvia Cardani 1,2, Tara A. Janes1,2, Silvia Pagliardini1,2,3
1Department of Physiology, 2Women and Children’s Health Research Institute, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada; 3Neuroscience and Mental Health Institute, University of Alberta, Edmonton, Canada

P21
Hypoxia-mediated increases in post-inspiratory drive require activity within the pontine respiratory group.
Rahat Ul Ain Summan Toor, Qi-Jian Sun, Cara M Hildreth, Jacqueline K Phillips & Simon McMullan
Macquarie Medical School, Macquarie University, NSW 2109 Australia

P22
ATP in the preBötzinger Complex acts via P2Y1Rs to potentiate Ih and attenuate the hypoxic ventilatory depression in anaesthetized adult rats
Vivian Biancardi1*, Yong Zhang1, Daniel B Zoccal1,2, Tucaauê S Alvares1, Carolina Scarpellini3, Silvia Pagliardini1, Gaspard Montandon3, Gregory D Funk1
1Dept of Physiology, NMHI, WCHRI, Univ of Alberta, Edmonton, Canada; 2Dept Physiology, Sao Paulo State Univ, Brazil; 3Keenan Research Centre Biomed. Science, St. Michael’s, Toronto, Canada

P23
Automated Approaches to Respiratory Studies
Savannah Lusk1, C. Ward1, A. Chang2, D. Patel, B. Ruiz2, M. Garcia Acosta3, A. Twitchell-Heyne1, S. Fattig1, G. Allen1,2, R. Ray1
1Baylor College of Medicine, 2Rice University, 3U. of Houston

P24
Functional characterization of abdominal expiratory activity during hypoxia and hypercapnia in unanesthetized rats
Isabela P. Leirão & Daniel B. Zoccal
Department of Physiology and Pathology, School of Dentistry, São Paulo State University, Araraquara, SP, Brazil

P25
Metabolic response, Ventilatory response, and Blood Lactate Concentration during the Incremental and Steady-State Running Tests in Young and Middle-aged Mice
Akira Yoshikawa1,2, Makito Iizuka1, Mitsuko Kanamaru1,3, Shotaro Kamijo1,4, Hirokazu Ohtaki5, Masahiko Izumizaki1
1Department of Physiology, Showa University School of Medicine. 2Division of Health Science Education Showa University School of Nursing and Rehabilitation Sciences. 3Faculty of Arts and Sciences at Fujiyoshida, Showa University. 4Department of Physiology, Showa University School of Pharmacy. 5Department of Functional Neurobiology, Tokyo University of Pharmacy and Life Sciences, School of Pharmacy

P26
Developing a genetically encoded sensor for CO2
Valentin-Mihai Dospinescu1, Nicholas Dale2
1 Warwick Medical School, University of Warwick, Coventry CV4 7AL, UK. 2 School of Life Sciences, University of Warwick, Coventry, CV4 7AL, UK.

Poster II (90 min) October 19 Wed (P27 – P50)

P27
Amygdala circuit involved in apnea induction in Scn1aR1407X/+ and Scn8aN1768D/+ mice, possible link to SUDEP
E Bravo1,2,4, MS Crotts1,2, BJ Dlouhy4,7, GB Richerson1,2,3,4,5,6
1Department of Neurology, 2The NINDS Center for SUDEP Research, 3Graduate Program in Neuroscience, 4The Iowa Neuroscience Institute, 5Department of Molecular Physiology and Biophysics, 6Department of Neurosurgery, University of Iowa, 7Veteran Affairs Medical Center, Iowa City, USA.

P28
Involvement of cannabinoid receptors in descending inhibition on spinal seizure-like activity in the phrenic output
Shih Tien Lin, Makito Iizuka, Shunya Yoda, Hiroshi Onimaru, Masahiko Izumizaki
Department of Physiology, Showa University School of Medicine, Tokyo, Japan

P29
A brainstem peptide system activated at birth protects postnatal breathing
Yingtang Shi1, Daniel S. Stornetta1, Robert J. Reklow2, Alisha Sahu1, Yvonne Wabara1, Ashley Nguyen1, Keyong Li1, Yong Zhang2, Edward Perez-Reyes1, Rachel A. Ross3,4, Bradford B. Lowell3, Ruth L. Stornetta1, Gregory D. Funk2, Patrice G. Guyenet1 & Douglas A. Bayliss1
1 Department of Pharmacology, University of Virginia, Charlottesville, VA, USA. 2 Department of Physiology, Women & Children’s Health Research Institute, Neuroscience and Mental Health Institute, University of Alberta, Edmonton, Alberta, Canada. 3 Beth Israel Deaconess Medical Center, Harvard University, Boston, MA, USA. 4 McLean Hospital, Department of Psychiatry, Harvard Medical School, Belmont, MA, USA

P30
Depression of respiratory activity by aconitine and its recovery by tetrodotoxin or lidocaine in brainstem-spinal cord preparations from newborn rats
Shunya Yodaa, Hiroshi Onimaru a, Masahiko Izumizaki a
a Department of Physiology, Showa University, Tokyo 142-8555, Japan

P31
Obligatory and Accessory Respiratory Muscle Physiology in Early Dystrophic Disease
Aoife D. Slyne1, Amandine May1, Roisin Dowd1, Sarah E. Drummond1, David P. Burns1 & Ken D. O’Halloran1.
1Department of Physiology, School of Medicine, College of Medicine and Health, University College Cork, Cork, Ireland.

P32
Control of breathing in a mouse model of Duchenne muscular dystrophy
Ken D. O’Halloran
Department of Physiology, School of Medicine, College of Medicine & Health, University College Cork, Cork, Ireland.

P33
Clinical course of congenital central hypoventilation syndrome diagnosed in infant
Yohei Hasebe1),2),3), Yosuke Yamada4), Yuki Maebayashi3), Tamao Shinohara3), Atsushi Nemoto3), Atsushi Naito3)
1.Department of Pediatrics, Faculty of Medicine, University of Yamanashi, Japan. 2.Clinical Research Center, National Hospital Organization Murayama Medical Center, Tokyo, Japan. 3.Department of Neonatology, Yamanashi Prefectural Central Hospital, Yamanashi, Japan. 4.Tokyo Women’s Medical University Adachi Medical Center, School of Medicine, Tokyo, Japan

P34
Rhythmic ventilatory-like activity of frog spinal nerves that’s not: a putative central sympathetic pattern generator
Marina R. Sartori1, Brittney A. Herrington1, Connor Braun1, Elisa M. Fonseca1, Richard J.A. Wilson1
1 Hotchkiss Brain Institute and Alberta Children’s Hospital Research Institute; Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary, AB, T2N4N1. Canada.

P35
Axonal projection of the medullary expiratory neurons in the thoracic spinal cord of the cat
Kenta Kawamura1), Kazumasa Sasaki2), Sei-Ichi Sasaki3), Kazuhide Tomita1)
1) Department of Physical Therapy, Ibaraki Prefectural University of Health Sciences. 2) Department of Anatomy, Toho University. 3) Toyo Public Health College

P36
Do sympathetic Preganglionic Neurons (SPNs) serve as spinal cord pressure sensors?
*Fonseca EM1, *Tenorio-Lopes L1,2, Barioni N1, Incognito A1, Dutschmann M3, Wilson RJA1; *Shared 1st authors
1Dept. of Physiology and Pharmacology, Hotchkiss Brain Institute and Alberta Children’s Hospital Research Institute, Cumming School of Medicine, University of Calgary. 2Dept. Animal Morphology and Physiology, Sao Paulo State University (UNESP), Brazil. 3Florey Neurosciences Institutes, University of Melbourne, Australia

P37
Raphe-cervical excitatory interneuron circuitry in the dynamic regulation of breathing in health and spinal cord injury
Kajana Satkunendrarajah 1,2,3, Allison Brezenski2,3, Sean Schrank1,2, Christy Dolick1,2
1. Department of Neurosurgery, Medical College of Wisconsin, Milwaukee, WI, USA. 2. Clement J. Zablocki Veterans Affairs Medical Center, Milwaukee, WI, USA. 3. Department of Physiology, Medical College of Wisconsin, Milwaukee, WI, USA

P38
Acute and chronic laryngeal deficits following cervical spinal cord injury
Kimberly Iceman1, Michael Frazure1, Courtney Strecker1, Clint Greene1, Dena Howland1, 2, Teresa Pitts1
1Department of Neurological Surgery and Kentucky Spinal Cord Injury Research Center, College of Medicine, University of Louisville, Louisville, KY, United States of America; 2Research Service, Robley Rex VA Medical Center, Louisville, KY, United States of America

P39
Applying massively parallel deconvolution to reconstruct the multidimensional structure of the rhythmogenic preBotzinger complex region
Naohiro Koshiya, Tibin T John, Hidehiko Koizumi, Yonghua Chen, Ruli Zhang, Jeffrey C Smith
National Institute of Neurological Disorders and Stroke, National Institutes of Health (NIH), Bethesda, MD, USA.

P40
Cholinergic projections to the preBötzinger Complex
Vivian Biancardi1*, Xiaqiu Yang1*, Xiuqing Ding1, Dhruv Passi1, Gregory D. Funk1, Silvia Pagliardini1 *co-first authorship
1Dept of Physiology, NMHI, WCHRI, Univ of Alberta, Edmonton, Canada

P41
Cell responses of the ventrolateral medulla induced by PAR1 activation and changes in respiratory rhythm in newborn rat en bloc preparations
Hiroshi Onimarua, Isato Fukushib, Keiko Ikedac, Itaru Yazawad, Kotaro Takedae, Yasumasa Okadaf, Masahiko Izumizakia
aDept. Physiol, Showa Univ. Sch. of Med., Tokyo, Japan. bFaculty of Health Sci., Aomori Univ. of Health and Welfare, Aomori, Japan. cDept. Oral Physiol, Showa Univ. Sch. of Dent., Tokyo, Japan. dDept. Food & Nutrition, Kyushu Nutrition Welfare Univ, Fukuoka, Japan. eSchool of Health Sciences, Fujita Health Univ., Toyoake, Aichi, Japan. fClinical Res. Ctr., Murayama Med. Ctr., Musashimurayama, Tokyo Japan

P42
Burstlets underlie preBötzinger rhythm generation in vivo
Evgeny Bondarenko, Sufyan Asshad, Jack L. Feldman
Department of Neurobiology, DGSOM, UCLA, Los Angeles, California 90095-1763, USA

P43
We observed regional differences in the contribution of hydrogen sulfide in the medullary respiratory center to respiratory intensity and frequency.
Minako Okazaki1,2 and Tadachika Koganezawa1,3
1. Department of Neurophysiology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, Japan. 2. Graduate School of Comprehensive Human Sciences, University of Tsukuba, Tsukuba, Ibaraki, Japan. 3. Transborder Medical Research Center, University of Tsukuba, Tsukuba, Ibaraki, Japan

P44
Persistent sodium current in the preBötzinger Complex does not contribute to the inspiratory–related rhythm generation
Carlos Aparecido DA SILVA JUNIOR1, Cameron J. Grover1, Maria Cristina D. Picardo1, Christopher A. Del Negro1
1Department of Applied Science, William & Mary, Williamsburg, 23185, Virginia, USA.

P45
Predictions and experimental tests of a new biophysical model of the preBötzinger complex inspiratory oscillator
Hidehiko Koizumi1, Ryan S. Phillips2,3, Yaroslav I. Molkov4,5, Jonathan E. Rubin2,3, Jeffrey C. Smith1
1Cellular and Systems Neurobiology Section, NINDS, NIH, Bethesda, MD. 2Department of Mathematics, University of Pittsburgh, Pittsburgh, PA. 3Center for the Neural Basis of Cognition, Pittsburgh, PA. 4Department of Mathematics and Statistics, Georgia State University, Atlanta, GA. 5Neuroscience Institute, Georgia State University, Atlanta, GA

P46
The neuronal mechanism controlling respiratory rate by excitatory neurons within the solitary nucleus
Noriyuki Hama1, Yasumasa Okada2, Shigefumi Yokota3
1Department of Neural and Muscular Physiology, Shimane University School of Medicine, Izumo, Japan. 2Clinical Research Center, Murayama Medical Center, Tokyo, Japan. 3Department of Anatomy and Neuroscience, Shimane University School of Medicine, Izumo, Japan

P47
Sigh generation in preBötzinger Complex
Evgeny Bondarenko1, Yan Cui1,2, Jack L. Feldman1
1 Department of Neurobiology, DGSOM, UCLA, Los Angeles, California 90095-1763, USA 2 Department of Physiology, Chengdu Medical College, China

P48
Early postnatal development in the properties of inspiratory neurons in the pre-Bötzinger complex of mice
Yoshihiko Oke1, Fumikazu Miwakeichi2,3, Yoshitaka Oku1, Johannes Hirrlinger4,5, Swen Hülsmann6,7
1Division of Physiome, Department of Physiology, Hyogo Medical University, Japan. 2Department of Statistical Modeling, The Institute of Statistical Mathematics, Japan. 3Department of Statistical Science, School of Multidisciplinary Sciences, The Graduate University for Advanced Studies, Japan. 4Carl-Ludwig-Institute for Physiology, Faculty of Medicine, University of Leipzig, Germany. 5Department of Neurogenetics, Max Planck Institute for Multidisciplinary Sciences, Germany. 6Clinic for Anesthesiology, University Medical Center, Germany. 7Research Center for Nanoscale Microscopy and Molecular Physiology of the Brain, Germany.

P49
Unraveling the connectivity of the Post-inspiratory Complex (PiCo)
Luiz M. Oliveira1; Lely Quina¹; Ginny Wu²; Xiangming Xu²; Jan-Marino Ramirez1
¹Center for Integrative Brain Research, Seattle Children’s Research Institute, Seattle, Washington 98101.

P50
Hypothalamic respiration-synchronized rhythmic activity in the diencephalon-lower brainstem-spinal cord preparation
Isato Fukushi1,2, Yosuke Kono2,3, Shigefumi Yokota4, Kotaro Takeda2,5, Masashi Yoshizawa2,3, Yohei Hasebe2,3, Mieczyslaw Pokorski6, Yasumasa Okada2
1 Faculty of Health Sciences, Aomori University of Health and Welfare, Aomori, Japan. 2 Clinical Research Center, Murayama Medical Center, Musashimurayama, Japan. 3 Department of Pediatrics, Faculty of Medicine, University of Yamanashi, Chuo, Japan. 4 Department of Anatomy and Neuroscience, Shimane University School of Medicine, Izumo, Japan. 5 Faculty of Rehabilitation, School of Health Sciences, Fujita Health University, Toyoake, Japan. 6 Institute of Health Sciences, Opole University, Opole, Poland

P51
COVID-19 and silent hypoxemia in a minimal closed-loop model of the respiratory oscillator
Casey O. Diekman, 1 Peter J. Thomas, 2 Christopher G. Wilson, 3
1. Department of Mathematical Sciences, New Jersey Institute of Technology, University Heights, Newark NJ. 2. Department of Mathematics, Applied Mathematics and Statistics, Case Western Reserve University, 10900 Euclid Avenue, Cleveland OH. 3. Department of Pediatrics & Basic Sciences, Loma Linda University, Lawrence D. Longo, MD Center for Perinatal Biology, 11223 Campus St, Loma Linda CA